Journal: PLoS ONE

Article Title: Silver and Gold Nanoparticles Exposure to In Vitro Cultured Retina – Studies on Nanoparticle Internalization, Apoptosis, Oxidative Stress, Glial- and Microglial Activity

doi: 10.1371/journal.pone.0105359

Figure Lengend Snippet: Fluorescent immunostaining: Iba1 (green) and ED1 (red) for detecting microglial cells (DAPI, blue). In the NP-exposed retinas (20 nm Ag (G–I), 80 nm Ag (J–L), 20 nm Au (M–O), 80 nm Au (P–R)) as well as in the control retinas (A–C) the Iba1- and ED–positive cells were located in the IPL, INL and GCL in all groups (A–C). AgNO 3 -exposed retina displayed the same staining pattern, but with a stronger intensity especially for the ED1-staining (D–F). Graph shows numbers of microglial cells and data is given as mean ±SD (n = 4 explants/group). * p <0.05 compared to control. GCL = ganglion cell layer, INL = inner nuclear layer, IPL = inner plexiform layer, ONL = outer nuclear layer, OPL = outer plexiform layer. Scale bar equals 200 µm.

Article Snippet: Sections were then incubated with primary antibodies, rabbit anti-glial fibrillary acidic protein (GFAP, 1∶1500 DAKO Cytomation, Glostrup Denmark), rabbit anti- Iba1 (1∶200, WAKO, Japan), and rat anti-mouse ED1 (CD68, 1∶1000, Nordic Biosite, Sweden), 1∶1000, overnight at 4°C overnight, and thereafter detected by incubation in secondary antibodies for 2 h. Secondary antibodies included were Texas Red-conjugated donkey anti-rabbit antibody (1∶200; Abcam, Cambridge, UK), Alexa 488 goat anti-rabbit IgG (Molecular Probes) and Alexa 564 goat anti-rat (Molecular Probes).

Techniques: Immunostaining, Control, Staining

Journal: PLoS ONE

Article Title: Silver and Gold Nanoparticles Exposure to In Vitro Cultured Retina – Studies on Nanoparticle Internalization, Apoptosis, Oxidative Stress, Glial- and Microglial Activity

doi: 10.1371/journal.pone.0105359

Figure Lengend Snippet: A–D. Iba1-immunohistochemistry revealing microglial cells with ramified (A), intermediate (B), round (C) and amoeboid (D) morphology, respectively. Quantification of the fractions of microglia in three different stages of activation was performed: intermediate or “early activated” cells as judged from the morphology and co-expression of ED1 while round- and amoeboid morphologies are regarded as “late activated” cells. The graph shows the distribution of activation stages, normalized to total numbers of Iba1-positive cells/explant; values ±SD (n = 4 explants/group) are given. * p <0.05 compared to control. GCL = ganglion cell layer; INL = inner nuclear layer; ONL = outer nuclear layer. Scale bars equal 50 µm.

Article Snippet: Sections were then incubated with primary antibodies, rabbit anti-glial fibrillary acidic protein (GFAP, 1∶1500 DAKO Cytomation, Glostrup Denmark), rabbit anti- Iba1 (1∶200, WAKO, Japan), and rat anti-mouse ED1 (CD68, 1∶1000, Nordic Biosite, Sweden), 1∶1000, overnight at 4°C overnight, and thereafter detected by incubation in secondary antibodies for 2 h. Secondary antibodies included were Texas Red-conjugated donkey anti-rabbit antibody (1∶200; Abcam, Cambridge, UK), Alexa 488 goat anti-rabbit IgG (Molecular Probes) and Alexa 564 goat anti-rat (Molecular Probes).

Techniques: Immunohistochemistry, Activation Assay, Expressing, Control